Methods of Protein Microcharacterization

Biological Methods Methods of Protein Microcharacterization: A Practical Handbook, edited by John E. Shively, 1986 Experiments in Molecular Biology, edited by Robert J. Slater, 1986 Methods in Molecular Biology, edited by John M. Walker ...

Methods of Protein Microcharacterization

Milestones in the techniques and methodology of polypeptide structure determination include the determination of the sequence of insulin by Sanger in 1951 (I) and the introduction of the repeti tive degradation of proteins with phenylisothiocyanate by Edman in 1959 (2). The automation of Edman chemistry (3) played a major role in the determination of polypeptide structures. Important modifications of Edman chemistry include the solid-phase approach by Laursen in 1971 (4) and the use of modified Edman reagents such as 4-N, N-dimethylaminoazobenzene-4'-isothiocy- ate (DABITC) for manual sequencing by Chang et al. (5) in 1976. A second major breakthrough in the analysis of polypeptides was automated amino acid analysis described by Spackman et al. in 1958 (6). However, during the period from 1975 to 1980, it became increasingly clear that the amount of material required for struc tural analysis was more than could be easily isolated for the vast majority of proteins. The field was criticized for its lack of sensitive techniques for the analysis of growth factors, immune modulators, membrane receptors, and peptide hormones. In addition, very little had been done to modernize and improve the original instruments introduced in the mid-1960s. The first indications of improved instrumentation for Edman chemistry came from Wittmann-Liebold's laboratory (7), followed by the introduction of a "micro" sequencer by Hunkapiller and Hood in 1978 (8). The movement toward improved instrumentation culminated in the "gas"--Phase sequencer of Hewick et al. (9) in 1981

More Books:

Methods of Protein Microcharacterization
Language: en
Pages: 456
Authors: John E. Shively
Categories: Science
Type: BOOK - Published: 2008-02-22 - Publisher: Springer Science & Business Media

Milestones in the techniques and methodology of polypeptide structure determination include the determination of the sequence of insulin by Sanger in 1951 (I) and the introduction of the repeti tive degradation of proteins with phenylisothiocyanate by Edman in 1959 (2). The automation of Edman chemistry (3) played a major role
Fundamentals of Protein Biotechnology
Language: en
Pages: 328
Authors: Stein
Categories: Medical
Type: BOOK - Published: 2017-10-19 - Publisher: Routledge

Fills a gap between the existing studies of proteins, which tend to be highly technical and geared toward the practicing protein chemist, and biochemistry textbooks, which focus on general principles. Scientists cover a dozen topics by presenting fundamental principles, an overview, and the practica
Methods for Investigation of Amino Acid and Protein Metabolism
Language: en
Pages: 280
Authors: Antoine E. El-Khoury
Categories: Medical
Type: BOOK - Published: 2017-10-05 - Publisher: Routledge

Containing all the new as well as classical methodologies used in the investigation of amino acid and protein metabolism in human and animal models, this book is needed because of the dramatic increase in research in this field. There is no other book currently on the market that covers these
Methods in Protein Sequence Analysis · 1986
Language: en
Pages: 658
Authors: Kenneth A. Walsh
Categories: Science
Type: BOOK - Published: 1987-06-17 - Publisher: Springer Science & Business Media

Methods in Protein Sequence Analysis -1986 brings together reports of the most recent methodology available to protein chemists for studying the molecular detail of proteins. The papers in this volume constitute the proceedings of the Sixth International Conference on Methods in Protein Sequence Analysis, which was held at the University
Chemical Reagents for Protein Modification
Language: en
Pages: 352
Authors: Roger L. Lundblad
Categories: Medical
Type: BOOK - Published: 2020-03-11 - Publisher: CRC Press

First published in 1991, Chemical Reagents for Protein Modification, 2nd Edition provides a unique combination of theoretical and practical considerations for the use of chemical reagents for site-specific modification of proteins. The book is divided into three sections, with the first section describing general techniques, including information on the organic